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Consider the following statements about ...

Consider the following statements about Gram staining.
I. All bacteria whether Gram positive (+) or Gram negative (-) will stain bluish-purple when stained with crystal voilet.
II. Gram negative bacteria appears pink on staining with safranin.
III. Iodine in Gram staining acts as a mordant.
IV. Alcohol treatment is required after Crystal Voilet lodine (CVI) complex.
Choose the correct option.

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