You have been asked to make a temporary stained mount of onion peel. Write four precautions which you would take during the experiment.

Explanation

### Step-by-Step Solution:

1. **Careful Removal of Onion Peel**: When you start the experiment, the first step is to carefully remove the thin peel of the onion. It is crucial to do this gently to avoid tearing the peel, as a torn specimen will not allow for a clear view of the cell structures under the microscope.

2. **Use of Distilled Water**: After obtaining the onion peel, immediately place it in distilled water. This step is important to keep the peel hydrated. If the peel dries out, it can lead to the formation of air bubbles and make it difficult to observe the plasma membrane clearly.

3. **Proper Staining**: When applying the stain (such as saffron), ensure that you use the correct amount—approximately 2-3 drops. Using too much or too little stain can affect the visibility of the cells. Allow the stain to sit for about 30 seconds to ensure proper absorption by the cells.

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