To answer the question regarding the seat of nucleases, proteases, lipases, and carbohydrates in a eukaryotic cell, we can follow these steps:
### Step-by-Step Solution:
1. **Understanding the Enzymes**:
- Nucleases are enzymes that break down nucleic acids by cleaving the phosphodiester bonds between nucleotides.
- Proteases are enzymes that digest proteins by breaking down peptide bonds.
- Lipases are enzymes that break down lipids (fats).
- Carbohydrases (or amylases) are enzymes that break down carbohydrates into simpler sugars.
2. **Identifying the Organelles**:
- We need to identify where these enzymes are primarily located within a eukaryotic cell. The options provided are:
- Vacuole
- Golgi complex
- Endoplasmic reticulum
- Lysosome
3. **Evaluating Each Option**:
- **Vacuole**: Primarily involved in storage and maintaining osmotic balance, not the site for these enzymes.
- **Golgi Complex**: Involved in modifying, sorting, and packaging proteins and lipids, but not primarily the site for nucleases, proteases, lipases, and carbohydrates.
- **Endoplasmic Reticulum (ER)**: Involved in the synthesis of proteins and lipids, but not the site for the breakdown of these macromolecules.
- **Lysosome**: Contains hydrolytic enzymes (digestive enzymes) that break down various biomolecules, including nucleic acids, proteins, lipids, and carbohydrates.
4. **Conclusion**:
- The correct answer is **Lysosome** (Option 4), as it is the organelle that serves as the seat for nucleases, proteases, lipases, and carbohydrates in eukaryotic cells.
### Final Answer:
The seat of nucleases, proteases, lipases, and carbohydrates in the eukaryotic cell is the **Lysosome**.
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