To determine the content of DNA in a chromosome at different phases of the cell cycle, we start with the given information that the DNA content is 2C after the M-phase. We will analyze the DNA content during the G1 phase, S phase, and Anaphase of mitosis step by step.
### Step-by-step Solution:
1. **Content of DNA after M-phase**:
- At the end of the M-phase (mitosis), the DNA content is given as 2C. This means that each chromosome has a complete set of DNA, and the cell is ready to enter the next phase of the cell cycle.
2. **Content of DNA in G1 phase**:
- After the M-phase, the cell enters the G1 phase. During G1, the cell grows and synthesizes RNA and proteins, but DNA replication has not yet occurred. Therefore, the DNA content remains the same as it was at the end of the M-phase.
- **Answer**: The DNA content in G1 phase is **2C**.
3. **Content of DNA in S phase**:
- The S phase is where DNA replication occurs. During this phase, each chromosome is duplicated, resulting in two identical sister chromatids for each chromosome. Consequently, the DNA content doubles.
- **Answer**: The DNA content in S phase is **4C**.
4. **Content of DNA in Anaphase of mitosis**:
- Anaphase is a stage of mitosis where the sister chromatids are separated and pulled towards opposite poles of the cell. At this point, although the chromatids are separated, the total amount of DNA in the cell remains the same as it was at the end of the S phase before division. However, since we consider the DNA content per chromosome, each chromosome still has the original amount of DNA.
- **Answer**: The DNA content in Anaphase is **2C** (as each chromosome, now separated, still has the same amount of DNA as it did at the end of G1).
### Summary of DNA Content:
- G1 phase: **2C**
- S phase: **4C**
- Anaphase: **2C**
### Final Answer:
The content of DNA at G1 is 2C, at S is 4C, and at Anaphase is 2C.
