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The methodology, which has been most use...

The methodology, which has been most useful for investigating the Calvin cycle, is

A

Radioactive isotope technique

B

Inverted funnel experiment

C

Half leaf experiment

D

Flash light experimental technique

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The correct Answer is:
**Step-by-Step Text Solution:** 1. **Understanding the Calvin Cycle**: The Calvin cycle is a crucial part of photosynthesis where carbon dioxide is fixed into organic molecules. Investigating this cycle helps us understand how plants convert light energy into chemical energy. 2. **Identifying Methodologies**: There are several methodologies used to study the Calvin cycle. The question asks for the most useful one. 3. **Evaluating Options**: - **Option 1: Radioactive Isotope Technique**: This method involves using radioactive carbon (specifically carbon-14) to trace the path of carbon during photosynthesis. Calvin used this technique to determine how carbon from CO2 is incorporated into organic compounds like starch and sucrose. - **Option 2: Inverted Funnel Experiment**: This experiment demonstrates that oxygen is produced during photosynthesis but does not specifically investigate the Calvin cycle. - **Option 3: Half-leaf Experiment**: Conducted by scientist Moll, this experiment shows that CO2 is necessary for photosynthesis but does not delve into the specifics of the Calvin cycle. - **Option 4: Flashlight Experimental Technique**: This experiment illustrates phototropism, the growth of plants towards light, and does not relate to the Calvin cycle. 4. **Conclusion**: The most useful methodology for investigating the Calvin cycle is the radioactive isotope technique, as it directly tracks the incorporation of carbon into organic molecules during photosynthesis. **Final Answer**: The methodology which has been most useful for investigating the Calvin cycle is the **radioactive isotope technique**. ---
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