Chloroplasts are disrupted and the stroma separated from the lamellae. The isolated stroma will fix `C_(2)` if it is supplied with-

Options

A. Oxygen
B. Carotenoid
C. Light
D. ATP + NADPH

Answer

To solve the question regarding what the isolated stroma will need to fix carbon dioxide after chloroplast disruption, we can break down the process step by step:

### Step-by-Step Solution:

1. **Understanding Chloroplast Structure**:
- Chloroplasts consist of two main parts: the stroma (the fluid-filled space) and the thylakoid membranes (where light reactions occur).
- The stroma is where the dark reactions (Calvin cycle) take place, which are responsible for fixing carbon dioxide into organic molecules.

2. **Disruption of Chloroplasts**:
- When chloroplasts are disrupted, the stroma is separated from the thylakoid membranes (lamellae). This means that the components necessary for light reactions (like chlorophyll) are no longer available to produce ATP and NADPH.

3. **Role of ATP and NADPH**:
- The dark reactions of photosynthesis (Calvin cycle) require energy in the form of ATP and reducing power in the form of NADPH, which are produced during the light reactions.
- Since the light reactions cannot occur in the isolated stroma, it cannot produce ATP and NADPH on its own.

4. **Requirement for Carbon Fixation**:
- For the isolated stroma to fix carbon dioxide, it must be supplied with ATP and NADPH. These molecules are essential for driving the reactions that convert carbon dioxide into glucose.

5. **Conclusion**:
- Therefore, the isolated stroma will fix carbon dioxide if it is supplied with **ATP and NADPH**.

### Final Answer:
The isolated stroma will fix carbon dioxide if it is supplied with **ATP and NADPH**.

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