Isolation of genetic material from fungal cells does not involve the use of.

Options

A. agarose
B. chitinase
C. ethanol
D. water

Answer

To answer the question "Isolation of genetic material from fungal cells does not involve the use of," we will analyze the options provided and determine which one is not used in the isolation process.

### Step-by-Step Solution:

1. **Understand the Context**:
- Fungi are eukaryotic organisms, and their genetic material is DNA. The goal is to isolate DNA from fungal cells.

2. **Review the Options**:
- We need to evaluate each option to see if it is involved in the isolation of DNA from fungal cells.

3. **Option Analysis**:
- **Option 1: Agarose**:
- Agarose is primarily used in gel electrophoresis, a technique for separating DNA fragments based on size. It is not used for the actual isolation of DNA from cells but rather for separating DNA after it has been isolated. Therefore, agarose is not involved in the isolation process.

- **Option 2: Chitinase**:
- Chitinase is an enzyme that breaks down chitin, a component of the fungal cell wall. By degrading the cell wall, chitinase facilitates the release of DNA from the fungal cells, making it essential for the isolation process.

- **Option 3: Ethanol**:
- Ethanol is used in the precipitation of DNA after it has been isolated. It helps in separating DNA from other cellular components by causing the DNA to precipitate out of solution. Thus, ethanol is involved in the isolation process.

- **Option 4: Water**:
- Water is often used in various steps of the isolation process, including diluting ethanol or as a solvent for other reagents. It is also essential for the overall extraction process.

4. **Conclusion**:
- Based on the analysis, the option that is not involved in the isolation of genetic material from fungal cells is **Option 1: Agarose**.

### Final Answer:
Isolation of genetic material from fungal cells does not involve the use of **agarose**.