If gene of interest was inserted at Sal I site in pBR322 the resulting will confer resitsacne to .

Options

A. Ampicilin
B. Tetracycline
C. Kanamycin
D. Both (1) &(3)

Answer

To solve the question regarding the insertion of a gene of interest at the Sal I site in the plasmid pBR322 and the resulting resistance conferred, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Context**: pBR322 is a well-known plasmid used in molecular biology. It contains genes that confer resistance to two antibiotics: ampicillin (Amp) and tetracycline (Tet).

2. **Identify the Sal I Site**: The Sal I restriction enzyme cuts DNA at specific sequences. In the context of pBR322, inserting a gene of interest at the Sal I site means that this site will be occupied by the new DNA.

3. **Determine the Effect of Insertion**: When a gene is inserted into a plasmid, it can disrupt the function of existing genes. In pBR322, if the gene of interest is inserted at the Sal I site, it can interfere with the tetracycline resistance gene.

4. **Analyze the Resistance**:
- If the tetracycline resistance gene is disrupted due to the insertion, the plasmid will still retain its ampicillin resistance.
- Therefore, the presence of the gene of interest at the Sal I site will lead to the loss of tetracycline resistance while maintaining ampicillin resistance.

5. **Conclusion**: The resulting plasmid after the insertion of the gene of interest at the Sal I site in pBR322 will confer resistance to ampicillin.

### Final Answer:
The resulting plasmid will confer resistance to **ampicillin**.