Which of the following is not a restriction endonuclease.

Options

A. Bam HI
B. Ori
C. Rop
D. Cla I

Answer

To determine which of the following is not a restriction endonuclease, we need to understand what restriction endonucleases are and their functions.

### Step-by-Step Solution:

1. **Definition of Restriction Endonucleases**:
- Restriction endonucleases, also known as restriction enzymes, are proteins that cut DNA at specific sequences. These sequences are often palindromic, meaning they read the same forwards and backwards.

2. **Function of Restriction Endonucleases**:
- They recognize specific palindromic sequences in DNA and cleave the DNA at these sites, creating "sticky ends" that can be used for DNA ligation with vectors.

3. **Identify the Options**:
- In the context of the question, we need to identify the options given (though not provided here) and determine which one does not fit the definition of a restriction endonuclease.

4. **Analyzing the Options**:
- For example, if we consider BAMHI and LAL as options, both are known restriction enzymes. However, if we encounter "RO", it is mentioned in the transcript as the "origin of replication" and not a restriction enzyme.

5. **Conclusion**:
- Based on the analysis, the answer to the question "Which of the following is not a restriction endonuclease?" would be the option that is identified as the origin of replication (RO).

### Final Answer:
- The option that is not a restriction endonuclease is **RO** (origin of replication).