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The sequence of a gene, which controls t...

The sequence of a gene, which controls the copy number of the linked DNA, in PBR 322 is

A

Bam HI

B

Ori

C

Rop

D

Cla I

Text Solution

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The correct Answer is:
To answer the question regarding the sequence of a gene that controls the copy number of the linked DNA in PBR 322, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding PBR 322**: - PBR 322 is a plasmid that serves as a vector for cloning DNA. It is important to know that plasmids are circular DNA molecules that can replicate independently of chromosomal DNA in bacterial cells. 2. **Identifying the Copy Number Control**: - The copy number refers to how many copies of the plasmid exist within a single bacterial cell. This is crucial for the efficiency of gene cloning and expression. 3. **Key Components of PBR 322**: - PBR 322 contains several important regions, including: - **ORI (Origin of Replication)**: This is where replication begins but does not directly control the copy number. - **Tetracycline Resistance Gene**: This allows for the selection of transformed cells but does not control copy number. - **ROP (Replication of Plasmid)**: This gene is responsible for the regulation of the plasmid's replication process. 4. **Conclusion**: - The gene that controls the copy number of the linked DNA in PBR 322 is the **ROP gene**. It codes for a protein that regulates the replication of the plasmid, thereby influencing how many copies of the plasmid are present in the host cell. ### Final Answer: The sequence of the gene that controls the copy number of the linked DNA in PBR 322 is the **ROP gene**. ---
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