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Identify the restrcition site present...

Identify the restrcition site present in a structural gene other than antibiotic resistant gene in pB322.

A

Bam HI

B

Pst I

C

Sal I

D

Pvu II

Text Solution

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The correct Answer is:
To identify the restriction site present in a structural gene other than the antibiotic-resistant gene in pBR322, follow these steps: ### Step-by-Step Solution: 1. **Understand the Context**: pBR322 is a plasmid used in E. coli that contains genes for antibiotic resistance (to ampicillin and tetracycline) and various restriction sites for cloning purposes. 2. **Identify the Antibiotic Resistance Genes**: The plasmid has two antibiotic resistance genes: - Tetracycline resistance (TET) - Ampicillin resistance (AMP) 3. **List the Known Restriction Sites**: The restriction sites associated with the antibiotic resistance genes are: - For Tetracycline: BamHI and SalI - For Ampicillin: PstI and BglII 4. **Look for Other Structural Genes**: The question asks for a restriction site in a structural gene that is not related to antibiotic resistance. 5. **Identify the ROP Gene**: The ROP gene (Replication of Plasmid) is important for controlling the replication of the plasmid in the host. 6. **Find the Restriction Site in ROP**: The restriction site associated with the ROP gene is PVU2. 7. **Conclusion**: The restriction site present in a structural gene other than the antibiotic-resistant gene in pBR322 is **PVU2**. ### Final Answer: The restriction site present in a structural gene other than the antibiotic-resistant gene in pBR322 is **PVU2**.
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