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Restriction enzyme belong to a large c...

Restriction enzyme belong to a large class of enzymes namely nucleases which are a type of

A

hdrolass

B

lyases

C

ligases

D

isomerase

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### Step-by-Step Solution: 1. **Understanding Restriction Enzymes**: - Restriction enzymes are proteins that cut DNA at specific sequences. They are crucial tools in molecular biology for gene cloning and DNA manipulation. 2. **Identifying the Class of Enzymes**: - Restriction enzymes are categorized under a larger class of enzymes known as nucleases. Nucleases are enzymes that cleave the bonds between nucleotides in nucleic acids (DNA and RNA). 3. **Defining Nucleases**: - Nucleases can be further classified into two main types: exonucleases and endonucleases. Restriction enzymes are a type of endonuclease, as they cut within the DNA strand rather than at the ends. 4. **Understanding the Function of Nucleases**: - Nucleases function by hydrolyzing the phosphodiester bonds between nucleotides, which is a chemical reaction that involves the addition of water (H2O). 5. **Classifying Nucleases**: - Since nucleases cleave nucleic acids using water, they are classified as hydrolases. Hydrolases are enzymes that catalyze the hydrolysis of various bonds, including those in nucleic acids. 6. **Conclusion**: - Therefore, the correct answer to the question is that restriction enzymes, which are nucleases, belong to the class of enzymes known as hydrolases. ### Final Answer: Restriction enzymes belong to a large class of enzymes namely nucleases, which are a type of hydrolase.
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