Which plasmid created by artifical method has both , tetracyline , and ampicillin resistance genes to be used as a selectable market ?

Options

A. pBR 322
B. pUC
C. Hind III
D. YAC

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: We need to identify a plasmid that has been artificially created and contains both tetracycline and ampicillin resistance genes. These resistance genes are important for selecting transformed cells in genetic engineering.

2. **Identifying Plasmids**: Among the options provided, we need to analyze the characteristics of each plasmid mentioned:
- **PBR 322**: This plasmid is known to contain both an ampicillin resistance gene and a tetracycline resistance gene. It is widely used in molecular cloning.
- **PUC**: This plasmid contains an ampicillin resistance gene but does not have a tetracycline resistance gene.
- **HIND 3**: This is not a plasmid but a restriction enzyme used in molecular biology.
- **Yeast Artificial Chromosome (YAC)**: This is a vector used for cloning in yeast cells and does not specifically contain the resistance genes mentioned.

3. **Conclusion**: Based on the analysis, the plasmid that contains both the tetracycline and ampicillin resistance genes is **PBR 322**.

4. **Final Answer**: The plasmid created by artificial methods that has both tetracycline and ampicillin resistance genes is **PBR 322**.

---