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Use of PEG to enable a host cell to ...

Use of PEG to enable a host cell to uptake a foregin DNA is known as :

A

Gene gum

B

Chemcial mediated transfer

C

electrophoresis

D

Microinjection

Text Solution

AI Generated Solution

The correct Answer is:
To answer the question regarding the use of PEG (polyethylene glycol) to enable a host cell to uptake foreign DNA, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Context**: The question is asking about a method that uses PEG to facilitate the uptake of foreign DNA into a host cell. PEG is a chemical that can help in the transformation process. 2. **Identify the Methods**: The question provides three options: Gene Gun, Electrophoresis, and Microinjection. We need to evaluate each method to see if they involve PEG. 3. **Evaluate Gene Gun**: The Gene Gun technique involves firing DNA-coated particles into cells. This method does not use PEG. It utilizes physical force to deliver DNA, so it is not the correct answer. 4. **Evaluate Electrophoresis**: Electrophoresis is a technique used for separating and isolating DNA fragments based on size and charge. It does not involve the uptake of DNA into cells, nor does it use PEG. Therefore, this option is also incorrect. 5. **Evaluate Microinjection**: Microinjection involves directly injecting DNA into the nucleus of a cell using a fine pipette. This method does not utilize PEG either, as it relies on mechanical means to introduce DNA into the cell. 6. **Identify the Correct Method**: The use of PEG for enabling the uptake of foreign DNA into host cells is known as "chemical-mediated transformation." This method does not involve any biological agents and directly incorporates DNA into the cells using the chemical properties of PEG. 7. **Conclusion**: The correct answer to the question is that the use of PEG to enable a host cell to uptake foreign DNA is known as "chemical-mediated transformation." ### Final Answer: The use of PEG to enable a host cell to uptake foreign DNA is known as **chemical-mediated transformation**. ---
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