Find the incorrec match with respect to insertion of recombinant DNA

Options

A. Micro injection method - Animal cells
B. Gene gun method - Plant cell
C. Disarmend Retroviruses - Animals Cells
D. Disarmed Tiplasmid - All angiosperms

Answer

To solve the question "Find the incorrect match with respect to insertion of recombinant DNA," we will analyze each option provided and determine which one is incorrect.

### Step-by-Step Solution:

1. **Analyze the First Option: Microinjection Method for Animal Cells**
- The microinjection method involves using a fine needle to inject the desired genetic material directly into the nucleus of the target animal cell. This method is indeed used for the insertion of recombinant DNA in animal cells.
- **Conclusion**: This option is correct.

2. **Analyze the Second Option: Gene Gun Method in Plant Cells**
- The gene gun method (or biolistics) involves coating gold or tungsten particles with the desired genetic material and then shooting these particles into plant cells at high velocity. This method is effective for transforming plant cells.
- **Conclusion**: This option is correct.

3. **Analyze the Third Option: Disarmed Retrovirus in Animal Cells**
- Disarmed retroviruses are commonly used as vectors to deliver recombinant DNA into animal cells. They can integrate their genetic material into the host genome, making them suitable for gene therapy and other applications.
- **Conclusion**: This option is correct.

4. **Analyze the Fourth Option: Disarmed Ti-plasmid for All Angiosperms**
- The Ti-plasmid (tumor-inducing plasmid) is derived from Agrobacterium tumefaciens and is used to transfer genes into plant cells, particularly dicots. However, it is not effective for all angiosperms, especially monocots (like rice and maize), which require different methods for transformation.
- **Conclusion**: This option is incorrect.

### Final Answer:
The incorrect match with respect to the insertion of recombinant DNA is **disarmed Ti-plasmid for all angiosperms**.

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