Identify the correct sequence of events in functioning of restriciton enzyme .
I. Finding specific recogntition sequence .
II. Binding to DNA .
III. Inspecting DNA .
IV . Cutting DNA .

Options

A. I,III,II,IV
B. III,II,I,IV
C. II,I,IV,III
D. II,I,III,IV

Answer

To identify the correct sequence of events in the functioning of a restriction enzyme, we can break down the process into four main steps:

1. **Finding Specific Recognition Sequence**: The restriction enzyme first locates the specific sequence of nucleotides on the DNA where it will act. This sequence is typically a palindromic sequence, which is recognized by the enzyme.

2. **Binding to DNA**: Once the recognition sequence is found, the restriction enzyme binds to the DNA at that specific site. This binding is crucial for the enzyme to perform its function.

3. **Inspecting DNA**: After binding, the enzyme inspects the DNA to confirm that it is at the correct recognition site. This step ensures that the enzyme is positioned correctly to make the cut.

4. **Cutting DNA**: Finally, the restriction enzyme cuts the DNA at the recognition site, resulting in either sticky or blunt ends, depending on the type of restriction enzyme.

Thus, the correct sequence of events is:
I. Finding specific recognition sequence
II. Binding to DNA
III. Inspecting DNA
IV. Cutting DNA