Home
Class 12
BIOLOGY
PCR involves...

PCR involves

A

DNA mutation

B

DNA amplificaiton

C

Gene signaling

D

Gene sequecing

Text Solution

AI Generated Solution

The correct Answer is:
**Step-by-Step Solution for the Question: "PCR involves"** 1. **Understanding PCR**: PCR stands for Polymerase Chain Reaction. It is a technique used to amplify specific segments of DNA, making multiple copies of a particular DNA sequence. 2. **Purpose of PCR**: The main purpose of PCR is DNA amplification. This means creating numerous identical copies of a specific DNA segment, which is essential for various applications in genetics, forensics, and medical diagnostics. 3. **Components of PCR**: The PCR process involves several key components: - **Template DNA**: The DNA that contains the target sequence to be amplified. - **Primers**: Short sequences of nucleotides that are complementary to the target DNA sequence. They provide a starting point for DNA synthesis. - **DNA Polymerase**: An enzyme that synthesizes new DNA strands by adding nucleotides to the primers. - **Nucleotides**: The building blocks of DNA that are required for the synthesis of new DNA strands. 4. **PCR Process**: The PCR process consists of three main steps that are repeated for 25 to 75 cycles: - **Denaturation**: The reaction mixture is heated to about 94-96°C, causing the double-stranded DNA to separate into two single strands. - **Annealing**: The temperature is lowered to allow the primers to bind (anneal) to their complementary sequences on the single-stranded DNA. - **Extension (Elongation)**: The temperature is raised again, and the DNA polymerase synthesizes a new DNA strand by adding nucleotides to the primers, resulting in the formation of new double-stranded DNA. 5. **Outcome of PCR**: After multiple cycles of denaturation, annealing, and extension, the result is the amplification of the target DNA sequence, producing millions of copies from a small initial sample. 6. **Conclusion**: The primary involvement of PCR is in **DNA amplification**, which allows scientists to obtain sufficient quantities of DNA for analysis and experimentation. ---
Promotional Banner

Similar Questions

Explore conceptually related problems

Consider the following statements with respect to PCR. A. PCR is now routinely used to detect HIV in suspected AIDS patients. B. PCR is being used to detect mutations in genes in suspected cancer patients. C. PCR involves the amplification of mRNA. D. In PCR repeated amplification of RNA is achieved by the use of a thermostable RNA polymerase. Of the above statement:

What is polymerase chain reaction (PCR) ? Explain in detail the steps involved in PCR.

What is PCR? Explain different steps involved in it.

Select the correct sequence of steps involved in PCR during gene amplification.

What does PCR stand for? Write its principle and name the different steps involved in PCR.

Identify the steps that are involved in PCR I. Denaturation II. Annealing III. Extension IV. Downstream processing

A: Taq Polymerase is involved in PCR technique. R: This enzyme remain active during the high temperature including denaturation of double stranded DNA.

Given figures represents the steps involved in polymerase chain reaction (PCR) . Identify the steps A,B,C and C and select the correct option.

The denaturation process in polymerase chain Reaction (PCR) does not involve