The bacterium used in the synthesis of human insulin artificially is

C-peptide of human insulin is

Some of the steps involved in production of humulin are given below. Choose the correct sequence (i) Synthesis of insulin gene artificially (ii) Culturing recombinant E. coli in bioreactors (iii) Purification of humulin (iv) Insertion and human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.

(i) Why was a bacterium used in the first instance of the construction of an artificial recombinant DNA molecule ? (ii) Name the scientists who accomplished this and how ?

Production of Human Insulin

The bacterium used in Griffith's experiment was :