Which of the following method is used for the detection of syphilis ?

Options

A. DNA - DNA hybridisation - PCR
B. Antibody detection - VDRL
C. Electron microscopy of culture
D. By gram staining of biopsy

Answer

**Step-by-Step Solution:**

1. **Understanding Syphilis**: Syphilis is a sexually transmitted disease caused by the bacterium Treponema pallidum. It can be transmitted through unprotected sexual intercourse or blood transfusion.

2. **Identifying Detection Methods**: The question asks for the method used to detect syphilis. Various methods can be used for the detection of different pathogens, including bacteria and viruses.

3. **Evaluating the Options**:
- **DNA-DNA Hybridization or PCR**: This method is primarily used for detecting viruses and involves amplifying small portions of DNA. It is not suitable for bacterial infections like syphilis.
- **Antibody Detection VDRL**: VDRL (Venereal Disease Research Laboratory) is a non-treponemal test used to detect antibodies produced in response to the Treponema pallidum bacteria. This method is specifically designed for syphilis detection.
- **Electron Microscopy of Culture**: This method is used for detecting viruses and involves studying samples under an electron microscope. It is not applicable for bacterial infections like syphilis.
- **Graham's Staining of Biopsy**: This method is used primarily for identifying cancerous or mutated cells and is not relevant for detecting syphilis.

4. **Conclusion**: Based on the evaluation of the options, the correct method for the detection of syphilis is the **Antibody Detection VDRL**.

5. **Final Answer**: The method used for the detection of syphilis is **Antibody Detection VDRL**.

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