Activation of Deoxyribonucleotides in DNA replication takes place by

Options

A. Ligase
B. Phosphorylase
C. Primase
D. Nuclease

Answer

**Step-by-Step Solution:**

1. **Understanding Deoxyribonucleotides**: Deoxyribonucleotides are the building blocks of DNA, consisting of a deoxyribose sugar, a phosphate group, and a nitrogenous base (adenine, guanine, cytosine, or thymine).

2. **Activation Process**: In DNA replication, deoxyribonucleotides need to be activated from their monophosphate form (dAMP, dGMP, dCMP, dTMP) to their triphosphate form (dATP, dGTP, dCTP, dTTP). This activation is crucial for DNA synthesis.

3. **Enzyme Involvement**: The enzyme responsible for this activation is called **phosphorylase**. This enzyme catalyzes the transfer of phosphate groups to convert the monophosphate forms into triphosphate forms.

4. **Role of ATP**: The activation process also involves ATP (adenosine triphosphate), which provides the necessary phosphate groups for the conversion.

5. **Conclusion**: Therefore, the correct answer to the question regarding the activation of deoxyribonucleotides in DNA replication is that it takes place by the action of the **phosphorylase enzyme**.

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