A hypothetical sequences from a transcription unit is represented below:
3'-ATGCATGCATGCATGCATGCATGC-5'
Template Strand
5'-TACGTACGTACGTACGTACGTACG-3'
Coding Strand
Write the sequence of RNA transcribed from above DNA ?

Options

A. 3'-AUGCAUGCAUGCAUGCAUGCAUGC-5'
B. 5'-AUGCAUGCAUGCAUGCAUGCAUGC-3'
C. 3'-UACGUACGUACGUACGUACGUACG-5'
D. 5'-UACGUACGUACGUACGUACGUACG-3'

Answer

To determine the RNA sequence transcribed from the given DNA template strand, we can follow these steps:

### Step-by-Step Solution:

1. **Identify the Template Strand**:
The template strand of DNA is given as:
5'-TACGTACGTACGTACGTACGTACG-3'

2. **Understand Transcription Direction**:
RNA is synthesized in the 5' to 3' direction, which means we will read the template strand in the 3' to 5' direction to create the complementary RNA strand.

3. **Complementary Base Pairing**:
The base pairing rules for transcription are as follows:
- Adenine (A) in DNA pairs with Uracil (U) in RNA.
- Thymine (T) in DNA pairs with Adenine (A) in RNA.
- Cytosine (C) in DNA pairs with Guanine (G) in RNA.
- Guanine (G) in DNA pairs with Cytosine (C) in RNA.

4. **Transcribe the Template Strand**:
Now we will transcribe the template strand from 3' to 5':
- 3'-TACGTACGTACGTACGTACGTACG-5' (Template Strand)
- 5'-AUGCAUGCAUGCAUGCAUGCAUG-3' (RNA Sequence)

5. **Final RNA Sequence**:
The RNA sequence transcribed from the given DNA template strand is:
5'-AUGCAUGCAUGCAUGCAUGCAUG-3'