" Number of restriction endonucleases of that can identify the recognition sequences of pBR322 is "

Write a note on recognition sequences or restriction sites.

pBR322 has restriction sites for many restriction endonucleases. Which of these enzymes acting on pBR322 is not correctly matched with its source ? I. Pvul : Providencia vulgaris. II. Clal: caryophanon latum. III. Sall : Streptomyces albus IV. Pstl: Proteus stuarti

Assertion : Each restriction endonuclease funtions by 'inspecting' the length of a DNA sequence Reason:Once it finds its specific recognition sequence, it will bind to the DNA and cut hydrogen bonds

Restriction endonuclease HindII acts on a palindromic specific sequence of ..........base pair.

A foreign gene is inserted in pBR322 using the restriction endonuclease EcoRl . Which of these is true about the E. coli having this pBR322 ?

Each restriction endonuclease functions by inspecting the length of a DNA sequence . It cleaves __________

Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at specific recognition sequence? What would be the disadvantage if they do not cut the DNA at specific recogniion sequence?

Restriction sites for Pvu I and Pvu II respectively are in which genes of pBR 322

Which of the following restriction endonucleases cannot be used while using pBR322 as a cloning vector ?

GAATTC is recognition site of restriction endonuclease