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The technique of transferring DNA fargme...

The technique of transferring DNA fargment separated on agarose gel to a synthetic membrane such as nitrocellulose is known as

A

Nothern blotting

B

Southern bolltting

C

Western boltting

D

Dot boltting

Text Solution

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The correct Answer is:
B
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Select the correct sequences of step in DNA finger printing involving Southern blot hybridisation using radiolabelled VNTR as probe. I. Hybridisation using labelled VNTR prode. II. Isolation of DNA. III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon. IV. Detection of hybridisation DNA fragments by autoradigraphy. V. Separation of DNA fragments by electrophoresis. VI. Digestion of DNA by restriction endonucleases.

Given below are the steps of DNA fingerprinting in random sequence:- (i) Isolation of DNA. (ii) Separation of DNA fragements by electrophoresis, (iii) Hybridisation using labelled VNTR probe. ltBrgt (iv). Transferring of separeted DNA fragments to synthetic membranes, such as nitrocellulose or nylon. (v) digestion of DNA by restriction endonucleases, (vi). Detection of hybridised DNA fragments by auto radio-graphy. which of the following is the correct sequence:-

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The technique of extraction of DNA fragments from the gel is called:

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The DNA fragments separated on an agarose gel can visualised after staining with

The DNA fragments separated on an agarose gel can be visualised after staining with