Which one in not a restriction enzyme :

Options

A. Eco `R_(1)`
B. Chitinase
C. Bam `H_(1)`
D. Hind -II

Answer

To determine which enzyme is not a restriction enzyme, we can follow these steps:

### Step 1: Understand what a restriction enzyme is
A restriction enzyme, also known as a restriction endonuclease, is an enzyme that cleaves DNA at specific recognition sites. These sites are typically palindromic sequences where the enzyme binds and cuts the DNA.

**Hint:** Look for the definition of restriction enzymes and their function in DNA cleavage.

### Step 2: Identify examples of restriction enzymes
Common examples of restriction enzymes include:
- EcoRI
- BamHI
- HindII (or HindI)

These enzymes are known for their ability to cut DNA at specific sequences.

**Hint:** Familiarize yourself with well-known restriction enzymes and their characteristics.

### Step 3: Analyze the options given in the question
In the question, we need to identify which enzyme is not a restriction enzyme. The options provided include:
- EcoRI
- BamHI
- HindII
- Chitinase

From our previous knowledge, EcoRI, BamHI, and HindII are all restriction enzymes.

**Hint:** Compare the listed options with the known examples of restriction enzymes.

### Step 4: Identify the non-restriction enzyme
Chitinase is mentioned as a hydrolytic enzyme that breaks down chitin by cleaving glycosidic bonds. It does not function as a restriction enzyme.

**Hint:** Look for enzymes that have different functions than cleaving DNA at specific sites.

### Conclusion
Based on the analysis, the enzyme that is not a restriction enzyme is **Chitinase**.

**Final Answer:** Chitinase is not a restriction enzyme.