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Assertion: Restriction endonucleases pro...

Assertion: Restriction endonucleases produces overhanging stretches called stricky ends on each DNA strand
Reason :Restriction enzymes cut the strand of DNA a little away from the centre of pallindrome sites

A

If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion

B

It both Assertion & Reason are True but Reason is not a correct explanation of the Assetion

C

If Assertion is True but the Reason is False

D

If both Assertion & Reason are false

Text Solution

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The correct Answer is:
A
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Restriction endonuclease ( EcoRI) enzyme cut the strand of DNA

Consider the following statements. A. Endonucleases remove nucleotides from the ends of the DNA whereas, exonucleases make cuts a specific positions within the DNA. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites. C. Asexual reproductions provides opportunities for variations and formulation of unique combinations of genetic setup. ltbrtgt D. DNA fragments can be separated by a technique known as gel electrophoresis. Of the above statements:

Assertion : Restriction enzymes cut the strand of DNA a little away from the centre of the palindromic sites Reason: Restriction enzymes cut the strads of DNA between the same two bases on the opposite strand.

The restriction endonuclease enzyme binds to the DNA and cuts

Assertion (A) : Restriction endonucleases recognise short pallindromic sequence and cut at specific sites Reason (R) : When a restriction endonuclease acts on a pallindrome, it cleaves both the strands of DNA molecule.

Assertion : Restriction endonuclease recognize short palindromic. Sequence and cut at specific sites. Reason : When a restriction endonuclease acts on Palindrome, it cleaves both the the strands of DNA molecules.

How many statements are correct? (a) Hind II always cut DNA molecules at particular point by recognizing a specific sequence of 6 bases. (b)After the restriction endonuclease action, single stranded portions which are overhanging stretches called sticky ends because they form nucleotide bonds with their complementary cut counter part. (c)The separated bands of DNA are cut out from the agarosegel and extracted from gel piece. This step is known as spooling. (d)Taq polymerase is used between annealing and extension of r-DNA technology.

Identify the restriction endonuclease that will provide sticky ends of DNA strands :

The 'sticky ends' on each of the two strands of a DNA generated by treatement with restriction enzyme facilitate the action of enzyme :