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Which of the following features are requ...

Which of the following features are required to facilitate cloning into a vector ?
a. Origin of replication (ori) b. Selectable marker c. Cloning site

A

a and b

B

b and c

C

a and c

D

a, b and c

Text Solution

Verified by Experts

The correct Answer is:
D

Origin of replication (ori), selectable marker and cloning site are required to faciliate cloning into a vector
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A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 2: Tools Of Tecombinant Dna Technology) Trestriction Enzymes ,
  1. In vecotr pBR 322, the 'rop' codes for th e

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  2. Alternate selectable markers have ability to differentiate recombinant...

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  3. Which of the following features are required to facilitate cloning int...

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  4. Which of the following are considered as useful selectable markers for...

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  5. The procedure through which a piece of DNA is introduced in a host bac...

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  6. DNA is stained by

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  7. Which is the palindromic nucleotide sequence ? {:((i) "GAATTC",(ii) ...

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  8. DNA fragments cut by restriction enzyme can be separated by

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  9. Read the following statements and find out the incorrect statements wi...

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  10. Which of the following bacteria is not a source of restriction endonu...

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  11. An antibiotic resistance gene in a vector usually helps in the selecti...

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  12. Which statement is incorrect regarding to restriction endonuclease enz...

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  13. Some foreign DNA fragment is attached to Cla I site of pBR 322. This r...

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  14. Selective markers in plasmids are used to

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  15. An enzyme catalysing the removal of nucleotides from the ends of DNA i...

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  16. BamH I site in present at

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  17. Selectable markers are present in

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  18. In pBR 322 amp^(R) and "tet"^(R) gene are present. When we ligate a fo...

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  19. DNA staining by ethidium bromide is followed by the exposure to UV ray...

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  20. Which is responsible for controlling the copy number of the linked DNA...

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