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Some foreign DNA fragment is attached to...

Some foreign DNA fragment is attached to Cla I site of pBR 322. This recombinant vector in used to transform Escherichia coli. Then on two different media, one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vecotr will

A

Grow on both, tetracycline and ampicillin containing media

B

Not grow on either tetracycline containing or ampicillin containing media

C

Grow on tetracycline but not on ampicillin containing medium

D

Grow on ampicillin but not on tetracycline containing medium

Text Solution

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The correct Answer is:
A
Some foreign DNA fragment is attached to Cla-I site of pBR322. This recombinant vector in used to transition Escherichia coli. Then on two different media, one containing ampicillin and the other containing tetracyline. The transformed cells containing the recombinant vector will grow on both, tetracyline and ampicllin containing media because Cla-I site is neither present on `amp^(R)` gene nor on `"tet"^(R)` gene
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A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 2: Tools Of Tecombinant Dna Technology) Trestriction Enzymes ,
  1. An antibiotic resistance gene in a vector usually helps in the selecti...

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  2. Which statement is incorrect regarding to restriction endonuclease enz...

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  3. Some foreign DNA fragment is attached to Cla I site of pBR 322. This r...

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  4. Selective markers in plasmids are used to

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  5. An enzyme catalysing the removal of nucleotides from the ends of DNA i...

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  6. BamH I site in present at

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  7. Selectable markers are present in

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  8. In pBR 322 amp^(R) and "tet"^(R) gene are present. When we ligate a fo...

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  9. DNA staining by ethidium bromide is followed by the exposure to UV ray...

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  10. Which is responsible for controlling the copy number of the linked DNA...

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  11. Restriction' in restriction enzyme refers to

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  12. The role of DNA ligase in the construction of a recombinant DNA molec...

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  13. Recognise the figure and find out the correct matching

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  14. Genetic engineering has been made possible due to

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  15. Plasmids are used in genetic engineering because they are

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  16. Which statement is incorrect

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  17. Which of the following is a plasmid ?

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  18. Genes for antibiotic resistance located in Or Bacterial resistance...

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  19. When a recombinant dNA is inserted within the coding sequencing of an ...

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  20. In the E.coli cloning vector pBR 322, the number of selectable marker ...

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