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In pBR 322 amp^(R) and "tet"^(R) gene ar...

In pBR 322 `amp^(R) and "tet"^(R)` gene are present. When we ligate a foreign DNA at Pst-I site, then recombinant plasmids will lose the resistance to the

A

Ampicillin

B

Tetracycline

C

Both A and B

D

None of the above

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The correct Answer is:
A
In pBR 322 `amp^(R) and "tet"^(R)` genes are present. When we ligate a foreign DNA at Pst-I site, then recombinant plasmids will lose the resistance to the ampicilin because when we ligate a foreign DNA at the Pst-I site of `amp^(R)` gene, the recombinant plasmid will close ampicillin resistance due to insertion of foreign DNA.
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A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 2: Tools Of Tecombinant Dna Technology) Trestriction Enzymes ,
  1. BamH I site in present at

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  2. Selectable markers are present in

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  3. In pBR 322 amp^(R) and "tet"^(R) gene are present. When we ligate a fo...

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  4. DNA staining by ethidium bromide is followed by the exposure to UV ray...

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  5. Which is responsible for controlling the copy number of the linked DNA...

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  6. Restriction' in restriction enzyme refers to

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  7. The role of DNA ligase in the construction of a recombinant DNA molec...

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  8. Recognise the figure and find out the correct matching

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  9. Genetic engineering has been made possible due to

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  10. Plasmids are used in genetic engineering because they are

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  11. Which statement is incorrect

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  12. Which of the following is a plasmid ?

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  13. Genes for antibiotic resistance located in Or Bacterial resistance...

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  14. When a recombinant dNA is inserted within the coding sequencing of an ...

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  15. In the E.coli cloning vector pBR 322, the number of selectable marker ...

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  16. Due to difficulty in inactivation of antibiotics, alternate selectable...

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  17. After the fragmentation by restriction endonuclease enzyme, the fragme...

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  18. In a chromosome there is a specific DNA sequence which is responsible ...

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  19. The separated bands of DNA are cut out from the agarose gel and extrac...

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  20. Restriction enzymes belong to a larger class of the enzyme called

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