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The role of DNA ligase in the constructi...

The role of DNA ligase in the construction of a recombinant DNA molecule is :

A

Formation of phosphodiester bond between two DNA fragments

B

Formation of hydrogen bonds between sticky ends of DNA fragments

C

Ligation of all purine and pyrimidine bases

D

All of the above

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The correct Answer is:
A
The role of DNA ligase in the construction of a recombinant DNA molecule is formation of phosphodiester bond between two DNA fragments
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A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 2: Tools Of Tecombinant Dna Technology) Trestriction Enzymes ,
  1. Which is responsible for controlling the copy number of the linked DNA...

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  2. Restriction' in restriction enzyme refers to

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  3. The role of DNA ligase in the construction of a recombinant DNA molec...

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  4. Recognise the figure and find out the correct matching

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  5. Genetic engineering has been made possible due to

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  6. Plasmids are used in genetic engineering because they are

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  7. Which statement is incorrect

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  8. Which of the following is a plasmid ?

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  9. Genes for antibiotic resistance located in Or Bacterial resistance...

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  10. When a recombinant dNA is inserted within the coding sequencing of an ...

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  11. In the E.coli cloning vector pBR 322, the number of selectable marker ...

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  12. Due to difficulty in inactivation of antibiotics, alternate selectable...

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  13. After the fragmentation by restriction endonuclease enzyme, the fragme...

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  14. In a chromosome there is a specific DNA sequence which is responsible ...

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  15. The separated bands of DNA are cut out from the agarose gel and extrac...

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  16. Restriction enzymes belong to a larger class of the enzyme called

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  17. In pBR 322, how many recognition sites are present ?

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  18. Presence of more than one recognition sites within the vector will

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  19. The stickiness of DNA ends faciliates the action of which enzyme

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  20. Ori is the some specific sequence in the vecotr pBR 322. Ori also has ...

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