Home
Class 12
BIOLOGY
Due to difficulty in inactivation of ant...

Due to difficulty in inactivation of antibiotics, alternate selectable markers developed to differentiate recombinants from non-recombinants on the basic of ability to

A

Produce bright orange colour in the prsence of a chromogenic substrate

B

Produce colour in the presence of a chromogenic substrate

C

Produce blue colour colonies due to insetional inactivation

D

All are correct

Text Solution

Verified by Experts

The correct Answer is:
B

Due to difficulty in inactivation of antibiotics, altermate selectable markers developed to differentiate recobinants from non-recombinants on the basis of ability to produce colour in the presence of a chromogenic substrate
Promotional Banner

Topper's Solved these Questions

  • BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

    A2Z|Exercise Section A: Topicwise Questions (topic 3: Processes Of Recombinant Dna Technology) Isolation Of The G|5 Videos
  • BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

    A2Z|Exercise Section A|1 Videos
  • BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

    A2Z|Exercise Section D: Chapter-end Test|50 Videos
  • BIOTECHNOLOGY AND ITS APPLICATION

    A2Z|Exercise Section C : PREVIOUS YEARS' EXAMINATIOIN QUESTIONS (Assertion and Reason Type Questions)|4 Videos
  • ECOSYSTEM

    A2Z|Exercise Section-d Chapter-end Test|50 Videos

Similar Questions

Explore conceptually related problems

Alternate selectable markers have ability to differentiate recombinants from non-recombinants due to the

In 'Lac' selection method of alternative selectable marker technique which of the following marker used for identification of recombinants from non recombinations (A) amp^(R) gene (B) tet^(R) gene (C) Lac z^(1) gene (D) x-gal

If PBR322 is cleaved with Bam HI to insert foreign DNA,which of the following selectable marker is used "],[" to screen recombinants from non- "],[" recombinants?

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

For selection of recombinants, insertional inactivation of antibiotic marker has been supercoded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

Characteristics OF Cloning Vectors:- 1.Origin OF Replication 2. Selectable Markers 3.Recognition (=cloning) Sites, selection OF non-transformants from transformants and Recombinants From Non-Recombinants, Insertional Inactivation

An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA helps in the selection of

Read the following statement regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option. I. Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. II. Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector. III. The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography. IV. A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection. {:(,I,II,III,IV),(1,"False","False","True","True"),(2,"True","True","False","False"),(3,"True","True","True","True"),(4,"False","False","True","False"):}

A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 2: Tools Of Tecombinant Dna Technology) Trestriction Enzymes ,
  1. When a recombinant dNA is inserted within the coding sequencing of an ...

    Text Solution

    |

  2. In the E.coli cloning vector pBR 322, the number of selectable marker ...

    Text Solution

    |

  3. Due to difficulty in inactivation of antibiotics, alternate selectable...

    Text Solution

    |

  4. After the fragmentation by restriction endonuclease enzyme, the fragme...

    Text Solution

    |

  5. In a chromosome there is a specific DNA sequence which is responsible ...

    Text Solution

    |

  6. The separated bands of DNA are cut out from the agarose gel and extrac...

    Text Solution

    |

  7. Restriction enzymes belong to a larger class of the enzyme called

    Text Solution

    |

  8. In pBR 322, how many recognition sites are present ?

    Text Solution

    |

  9. Presence of more than one recognition sites within the vector will

    Text Solution

    |

  10. The stickiness of DNA ends faciliates the action of which enzyme

    Text Solution

    |

  11. Ori is the some specific sequence in the vecotr pBR 322. Ori also has ...

    Text Solution

    |

  12. In pBR 322, recognition segqences, that are present on the same type o...

    Text Solution

    |

  13. The following figure shows

    Text Solution

    |

  14. Restriction endonucleases used widely in RDT are obtained from

    Text Solution

    |

  15. Construction of recombinant DNA involves

    Text Solution

    |

  16. Identify the vector suitable for cloning long DNA fragments

    Text Solution

    |

  17. Nucleic acid is fragmented by enzyme

    Text Solution

    |

  18. Tumor inducing (Ti) plasmid transforms :

    Text Solution

    |

  19. Restriction endonucleases are

    Text Solution

    |

  20. Plasmids are used as vectors in genetic engineering because of their

    Text Solution

    |