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The separated bands of DNA are cut out f...

The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is called

A

Electrophoresis

B

Resolution

C

Elution

D

Spooling

Text Solution

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The correct Answer is:
C
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is called elution.
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The separated bands of DNA are cut out from the agarose gel and are extracted from the gel piece. This is known as

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Study the given figure carefully and select the incorrect statements regarding this. (i) It represents a typical agarose gel electrophoresis in which lane 1 contains undigested DNA (ii) Smallest DNA bands are formed at A and largest DNA bands are formed at B (iii) The separated DNA fragments can be visualized after staining in the visible light (iv) The separated DNA bands are cut out from the agarose gel and extracted from the gel piece. this step is known as elution.

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out fron agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites, Ori, antibiotic resistance genes & ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

From where the agarose extracted ?

Moisturising gel is extracted from

A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 2: Tools Of Tecombinant Dna Technology) Trestriction Enzymes ,
  1. When a recombinant dNA is inserted within the coding sequencing of an ...

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  2. In the E.coli cloning vector pBR 322, the number of selectable marker ...

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  3. Due to difficulty in inactivation of antibiotics, alternate selectable...

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  4. After the fragmentation by restriction endonuclease enzyme, the fragme...

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  5. In a chromosome there is a specific DNA sequence which is responsible ...

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  6. The separated bands of DNA are cut out from the agarose gel and extrac...

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  7. Restriction enzymes belong to a larger class of the enzyme called

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  8. In pBR 322, how many recognition sites are present ?

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  9. Presence of more than one recognition sites within the vector will

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  10. The stickiness of DNA ends faciliates the action of which enzyme

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  11. Ori is the some specific sequence in the vecotr pBR 322. Ori also has ...

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  12. In pBR 322, recognition segqences, that are present on the same type o...

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  13. The following figure shows

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  14. Restriction endonucleases used widely in RDT are obtained from

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  15. Construction of recombinant DNA involves

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  16. Identify the vector suitable for cloning long DNA fragments

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  17. Nucleic acid is fragmented by enzyme

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  18. Tumor inducing (Ti) plasmid transforms :

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  19. Restriction endonucleases are

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  20. Plasmids are used as vectors in genetic engineering because of their

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