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Presence of more than one recognition si...

Presence of more than one recognition sites within the vector will

A

Facilitates the gene cloning

B

Facilitates the action of DNA ligase

C

Facilitates the action of restriction enzyme

D

Complicate the gene cloning

Text Solution

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The correct Answer is:
D
`**` To link the alien DNA the vector needs to have very few, prefecably single recognition sites for the commonly used restriction enzymes.
`**` Presence of more than one recognition site within the vector will generate several fragment which will complicate the gene cloning
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Read the given statements and select the correct option. Statement 1 : The cloning vector is required to have very few, preferably single, recongnition sites for the commonly used restriction enzymes. Statement 2: Presence of more than one recongnition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloining.

Read the following statements and select the correct ones. (i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes. (iii) Exonucleases make cuts at specific positions within the DNA. (iii) Hind ll was the first restriction endonuclease to be isolated. (iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA. (v) Presence of more than one recognition sites for a enzyme within the vector complicates the gene cloning.

Assertion: Presence of restriction sites of many enzymes within the vecotr is not desirable Reason :It will generate several fragments which will complicate the process

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A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 2: Tools Of Tecombinant Dna Technology) Trestriction Enzymes ,
  1. When a recombinant dNA is inserted within the coding sequencing of an ...

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  2. In the E.coli cloning vector pBR 322, the number of selectable marker ...

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  3. Due to difficulty in inactivation of antibiotics, alternate selectable...

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  4. After the fragmentation by restriction endonuclease enzyme, the fragme...

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  5. In a chromosome there is a specific DNA sequence which is responsible ...

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  6. The separated bands of DNA are cut out from the agarose gel and extrac...

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  7. Restriction enzymes belong to a larger class of the enzyme called

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  8. In pBR 322, how many recognition sites are present ?

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  9. Presence of more than one recognition sites within the vector will

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  10. The stickiness of DNA ends faciliates the action of which enzyme

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  11. Ori is the some specific sequence in the vecotr pBR 322. Ori also has ...

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  12. In pBR 322, recognition segqences, that are present on the same type o...

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  13. The following figure shows

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  14. Restriction endonucleases used widely in RDT are obtained from

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  15. Construction of recombinant DNA involves

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  16. Identify the vector suitable for cloning long DNA fragments

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  17. Nucleic acid is fragmented by enzyme

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  18. Tumor inducing (Ti) plasmid transforms :

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  19. Restriction endonucleases are

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  20. Plasmids are used as vectors in genetic engineering because of their

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