Find the correct match for the breaking of the cell wall during isolation of genetic material in rDNA procedure

Options

A. Cellulase -Plant cell
B. Lysozyme-Fungus
C. Chitinase-Bacteria
D. All of the above

Answer

A

Explanation

`**` In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules
`**` Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, protein, polysaccharides and also lipids
`**` This can be achieved by treating the bacterial cells/plant or animals tissue with enzymes such as:
(i) Lysozyme (bacteria)
(ii) Cellulase (plant cells)
(iii) chitinase (fungus)