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Small chemically synthesised oligonucleo...

Small chemically synthesised oligonucleotides that are complementary to the regions of DNA are called primers and present at the ....

A

5' end

B

3' end

C

Both 3' and 5' end

D

None of the above

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**Step-by-Step Solution:** 1. **Identify the Definition of Primers**: Small chemically synthesized oligonucleotides that are complementary to specific regions of DNA are known as primers. 2. **Understand the Nature of Primers**: Primers can be either short DNA or RNA molecules. They are specifically designed to bind to a target sequence of DNA. 3. **Recognize the Role of Primers**: Primers play a crucial role in various applications, particularly in genetic testing, research, and forensic science. They are essential for processes like PCR (Polymerase Chain Reaction), where they initiate the replication of a specific DNA segment. 4. **Define Oligonucleotides**: The term "oligonucleotide" refers to a short sequence of nucleotide residues. These sequences are typically made up of 20-30 nucleotides and are synthesized to be complementary to the target DNA sequence. 5. **Summarize the Importance of Primers**: In summary, primers are vital tools in molecular biology that facilitate the amplification and analysis of DNA, making them indispensable in genetic testing and other scientific research.

**Step-by-Step Solution:** 1. **Identify the Definition of Primers**: Small chemically synthesized oligonucleotides that are complementary to specific regions of DNA are known as primers. 2. **Understand the Nature of Primers**: Primers can be either short DNA or RNA molecules. They are specifically designed to bind to a target sequence of DNA. 3. **Recognize the Role of Primers**: Primers play a crucial role in various applications, particularly in genetic testing, research, and forensic science. They are essential for processes like PCR (Polymerase Chain Reaction), where they initiate the replication of a specific DNA segment. ...
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Read the following statements carefully and select the correct option :- (A) Source of the restriction enzyme Hind II is Haemophilus influenzae. (B) If any protein encoding gene is expressed in a heterologous host, it is called as recombinant protein (C) Microinjection method for injecting foreign gene is used for animal cell. (D) DNA primers are chemically synthesized oligonucleotides that are complementary to the regions of DNA in PCR. How many of the above statements are correct ?

Rearrange the following in the correct sequence to accomplish an important biotechnological reactions : (a) In vitro synthesis of copies of DNA of interest (b) Chemically synthesized oligonucleotides (C) Enzyme DNA-polymerase (d) complementary region of DNA (e) Genomic DNA template (f) Nucleotides provided (g) Primers (h) Thermostable DNA-polymers (from Thermus aquaticus) (i) Denaturation of ds-DNA

Rearrange the following in the correct sequences to accomplish an important biotechnological reaction: a) In vitro synthesis of copies of DNA of interest b) Chemically synthesized oligonucleotides. c) Enzymes DNA polymerase c) Complementary region of DNA. e) Genomic DNA template f) Nucleotides provided. g) Primers h) Thermostable DNA-polymerase (from Thermus aquaticus). i) Denaturatioin of ds-DNA.

The coding region on the DNA is called a

Small oligonucleotides capable of recognising complementary sequence are known as:

Rearrrange the following in the correct sequence to accomplish an important biotechnological reaction: ( a) In vitro synthesis of copies of DNA of interest (b) Chemically synthesized oligo - nucleqtides ( c) Enzyme DNA - polymerase ( d) Complementary region of DNA ( e) Gernomic DNA template (f) Nuceltides provided ( g)Primes (h) Thermostable DNA -polymerase (from Thermus aquaticus ) ( i) Denaturation of ds- DNA

Shallow water region present on the edge of lake is called as

The small pores present in the epidermis of the leaf are called

Chemicals synthesised by one organism that affect behaviour of another member of the same species are called

Which of these statements about protein synthesis are NOT correct ? I. During transcription, mRNA synthesized from DNA nucleotides to have the same sequence of nucleotides as the DNA strand on which it was made. II. During transcription , tRNA is synthesized from RNA nucleotides and carries codons that are complementary to the sequence of nucleotides on the DNA strand on which it was made. III. During translation, mRNA is synthesized from RNA nucleotides to have the complementary sequence of nucleotides to that. Of the DNA strand on which it was made . IV. During translation ribosomes move on mRNA from '5' and '3' one codon at a time .This is called translocation

A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 4: Amplification Of Gene Of Interest Using Pcr) Insertion Of R
  1. Which has the ability to transform normal cells into cancerous cell ?

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  2. Which is used as a cloning vecotr into plants ?

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  3. Which of the following statements is/are correct about heat shock

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  4. Which of the following steps are catalysed by Taq polymerase in a PCR ...

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  5. Significance of 'heat shock' method in bacterial transformation is to ...

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  6. Small chemically synthesised oligonucleotides that are complementary t...

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  7. Which of the following has popularised the PCR ?

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  8. Select the correct statement(s)

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  9. Select the correct statement(s)

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  10. Read the following statements (i) Primers used in PCR are small chem...

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  11. DNA polymerase enzyme used in PCR is isolated from

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  12. Thermostable enzymes 'Taq and Pfu' isolated from thermophilic bacteria...

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  13. Which is correctly matched?

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  14. Gene amplification using primers can be done by :

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  15. PCR proceeds in three distinct steps governed by temperature they are ...

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  16. Enzyme required for polymerase chain reaction (PCR) is

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  17. PCR technique was invented by

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  18. The source of Taq polymerase used in PCR is a :

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  19. Polymerase chain reaction employ

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  20. Polymerase chain reaction is most useful in

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