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Gel electrophoresis is used for...

Gel electrophoresis is used for

A

Construction of recombinant DNA by joining with cloning vectors

B

Isolation of DNA molecules

C

Cutting of DNA into fragments

D

Separation of DNA fragment according to their size

Text Solution

Verified by Experts

The correct Answer is:
D
Gel electrophoresis is used for separation of DNA fragments according to their size
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Knowledge Check

  • Gel electrophoresis is a

    A
    technique of separation of charged molecules under the influence of magnetic field
    B
    technique of incorpotation of DNA molecules into the cell through transient pores made due to electrical impulses
    C
    technique of separation of DNA fragments through the pores of agarose gel under the influence of electric field
    D
    technique of separation and purification of gene products.

  • Assertion (A) : Agarose gel electrophoresis is used to check the progression of a restriction enzyme digestion. Reason (R) : Restriction enzyme digestions are performed by incubating purified DNA molecules with restriction enzyme.

    A
    Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).
    B
    Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).
    C
    Assertion (A) is true but reason (R) is false.
    D
    Assertion (A) is false but reason (R) is true.

  • Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis.

    A
    (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect
    B
    (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect
    C
    (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect
    D
    (i) size, sieving effect (ii) agarose, eas weeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

    • Similar Questions

    Explore conceptually related problems

    Gel electrophoresis is employed in . I. Separation of DNA fragmetns . II. Selection of DNA fragments . III. Vissualizing DNA IV. Check the progression of restriction enzyme digestion.

    Most commonly used matrix in gel electrophoresis is

    The most commonly used matrix in gel electrophoresis is

    Separation of DNA fragments using agarose gel electrophoresis occurs due to:

    The most commonly used matrix in gel electrophoresis is agarose, which is a natural polymer extracted from :-

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