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Recombinant DNA bearing ampicillin resis...

Recombinant DNA bearing ampicillin resistance gene is passed in E. coli. The latter are spread on agar plates containing ampicillin. Then

A

Both transformed and untransformed cells die

B

both transformed and untransformed cells grow

C

Transformed recipient cells grow and untrasformaed cells die

D

Transformed recipient cell die and untransformed cells grow

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The correct Answer is:
C
Recombinant DNA bearing ampicillin resistance gene is passed in E.coli. The latter are spread on agar plantes containing ampicillin. Then transformed recipient cells grow and untransformed cells die
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If a recombinant DNA bearing gene for ampicillin resistance if transferred into E.Coli cells and the host cells are spread on agar plates containing ampicillin, then:

Assertion: The ampicillin resistance gene is called as selectable marker gene Reason :If host cells are spreaded on agar plates contraining ampicillin and only untransformed cells will survive

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as

(a) Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it. (b) What does the ampicillin resistant gene act as in the above case?

Assertion : Selectrable markers helps in identifying and eliminating non-transformants and selectively permitting the growth of transformants Reason: If a recombinant DNA bearing gene for resistance to ampicillin is transferred into E.coli cells, the host cells become transformed into ampicillin-resistant cells

Read the following statements and select the incorrect ones. (i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells with grow. (ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules. (iii) Downstream processing is one of the steps of rDNA technology. (iv) Disarmed pathogen vectors are also used in transfer of rDNA into the host.

A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section D: Chapter-end Test
  1. In genetic engineering , restriction enzymes are used for cutting

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  2. Melting of DNA at 70^(@)C is due to breakdown of

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  3. Fragments of DNA formed after treatment with endonucleases are separat...

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  4. Plasmids are suitable vectors for gene cloning because they are

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  5. Identify the plasmid

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  6. Autonomously replicating circular extrachromosomal DNA is called

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  7. In recombinant DNA technology, the term vector refers to

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  8. Vector for T-DNA is

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  9. What is true of plasmid

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  10. T(1) plasmid is used for making transgenic plants. It is obtained from

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  11. Tumour inducing plasmid used in producing transgenic plants is that of

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  12. In gel electrophoresis, differential mobility of DNA depends upon

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  13. Restriction enzymes are also called

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  14. The chemical knives of DNA are Or Enzyme that cleaves nucleic ac...

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  15. Which one of the following palindromic base sequences in DNA can be ea...

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  16. The enzyme capable of cutting DNA molecule at specific sites is

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  17. Biolistic technique is used in :

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  18. The ends of DNA fragments are stickly due to

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  19. Recombinant DNA bearing ampicillin resistance gene is passed in E. col...

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  20. The most extensively used bacteria in genetic engineering is

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