Explain the steps involved in the production of genetically engineered insulin .

Explanation

(i) Insulin consists of two polypeptide chains. Chain A and chain B . The gaens for these chains are isolated .
(ii) Then using restriction enzymes the genes for these chains and vector DNA are left at specific point .
(iii) These genes are then inserted into the vector DNA plasmid and joined using the ligase enzyme.
(iv) Subsequently then recombinant DNA replicates inside the E. Coli plasmid.
(v) The chains A and B are then extracted from the E . Cole plasmid.
(vi) These chains are then joined by the disulphide bonds to produce the humen insulin.