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Explain the procedure by which PCR aids ...

Explain the procedure by which PCR aids in early detection of cancer.

Text Solution

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• A single stranded DNA or RNA is tagged with a radioactive molecule(probe)
• It is allowed to hybridize to its complementary DNA in a clone of cells followed by detection using autoradiography.
• The clone having the mutated gene will hence not appear on the photographic film,
• because the probe will not have complementarity with the mutated gene. Hence, cancer induced mutation can be detected.
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