रिकम्बिनेन्ट डी एन ए टेक्नोलॉजी से इन्सुलिन कैसे बनाया जाता है?

Explanation

Production of newer and safer therapeutic drugs, called recombinant drugs. Insulin, a pancreatic horinone, used for conversion of excess glucose into glycogen. Its deficiency causes diabetes mellitus. The effort of synthesis of human insulin through recombinant DNA technology was done by Eli lily 1983. Two sequence of DNA was prepared for the two chain, A (21 amino acid) and B (30 amino acid) of insulin by reverse transcription of their mRNA. Plasmnid of E.coli and insulin gene are treated with same restriction endonuclease to produce sticky end and joined together by DNA Ligase recombinant DNA in form of plasmid carrying insulin genes