What are sticky ends and how are they created ?

What are sticky ends? State their significance in recombination DNA technology.

How are 'sticky ends' formed on a DNA strand? Why are they so called ?

Sticky ends, observed in the DNA , are due to the action of a particular type of nucleases. Which of the following statements is correct regarding the sticky ends of the DNA strand ?

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

The 'sticky ends' on each of the two strands of a DNA generated by treatement with restriction enzyme facilitate the action of enzyme :

Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : In this process, there is net gain of twenty four molcules of ATP.

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