Explain briefly :
(a) Restriction enzymes
(b) Recombination DNA

Explain briefly (a) PCR (b) Restriction enzymes and DNA (c) Chitinase

" DNA of bacterium is not cleaved by its own restriction enzymes because the recognition DNA requences are "

The role of restriction enzymes in DNA technology is to

A: Restriction enzymes belong to a larger class of enzymes called nucleases. R: Each restriction enzymes recognises sequence in the DNA.

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

Assertion (A) : Agarose gel electrophoresis is used to check the progression of a restriction enzyme digestion. Reason (R) : Restriction enzyme digestions are performed by incubating purified DNA molecules with restriction enzyme.

Restriction enzyme EcoR I cleavages DNA at the sequence