Unless the vector and source DNA are cut, fragments separated and joined, the desired recombinant vector molecule cannot be created.
(a) How are the desirable DNA sequence cut ?
(b) Explain the technique used to separate the cut fragments.
(c) how are the resultant framents joined to the vector DNA molecule ?

Unless the vector and source DNA are cut, fragments separated and joined, the desired recombinant vector molecule cannot be created. (a) How are the desirable DNA sequences cut ? (b) Explain the technique used to separate the cut fragments. (c) How are the resultant fragments joined to the vector DNA molecule ?

How are the desired DNA sequence used in biotechnology cut? Explain the technique used to separate the cut fragments.

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

Name and explain the techniques used in separation and isolation of DNA fragments to be used in recombinant DNA technology.

How are the DNA fragments separated and isolated for DNA fingerprinting? Explain.

A fragment of DNA, cut by a restriction enzyme, forms bonds with other DNA molecules that have

Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

Name and explain the technique that helps in the separation of DNA fragments for DNA recombinant technology experiments. How can these separated DNA fragments be visualised ?

DNA fragment with gene of interest and vector DNA are joined together by action of

DNA fragments cut by restriction enzyme can be separated by