Draw pBR 322 cloning vector. Lebel 'ORI', _'ROP' and any one antibiotic resistance site on it and state their functions.

In genetic engineering antibiotic resistance is often cloned into a vector to :

E. coli cloning vector pBR322 showing restriction sites ori and antibiotic resistance genes is given below. Select the correct option .

Which antibiotic resistance genes will you find in the modified vector pBR322?

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites, Ori, antibiotic resistance genes & ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out fron agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants ?