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The cell which contain recombinant DNA...

The cell which contain recombinant DNA

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Transformed and recombinant cell
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A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake. An exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e., bacterial trasformation?

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Some foreign DNA fragment is attached to Cla I site of pB322. This recombinant vector is used to transit Escherichia coli host cells. The cell subjected to transformation are plated on two different media-one containing ampicillin and the other containing tetracycline. THe transformed cells containing the recombinant vector

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Arrange the following steps for making the bacterial cell competent to take up DNA, in chromonological order . I. Incubation of cells with recombinant DNA on ice. II . Incubation of cells with recombinant DNA at 42 degree Celsius. III. Treating cells with a specific concentration of a divalent cation such as calcium .

Which of the followings is first recombinant DNA produced unintentionlly ?