True and false :
In insertional inactivation process alien gene introduced in ori. Sequence.

In the process of insertional inactivation

For selection of recombinants, insertional inactivation of antibiotic marker has been supercoded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

After insertion inactivation of galactosidase gene which type of colonies do not produce any colour ?

Assertion: The inactivation of any vector gene due to insertion of desired gene called as insertional inactivation Reason :Some time restriction sites are also present in between the gene sequence of vector

True and false : The uptake of DNA furing transformation is an active process.

True and false : In recombinant DNA, DNA inserted within the coding sequence of an enzyme beta galactosidase.

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introducting of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

Tools OF Genetic Engineering, Competent Host, Types OF Gene Transfer:-a)Vector Mediated Transfer,b) Vector Less Transfer ,Insertional Inactivation, Selecting Transformant and Non-Transformant and Between Recombinant and Non-Recombinant

Tools OF Genetic Engineering || Competent Host || Types OF Gene Transfer:-a)Vector Mediated Transfer || Vector Less Transfer || Insertional || Inactivation || Selecting Transformant and Non-Transformant || Recombinant and Non-Recombinant

Three basic steps are required for making GMO are : I. Identification of DNA with a desirable gene . II. Introduction of identified DNA into the host . III. Maintenance of introduced DNA to its progeny . What is the correct sequence of involvement ?