Rashid isolated a natural plasmid from a bacterium and is planning to facilitate cloning.
a. What are the minimum requirements for considering the isolated plasmid as a vector?
b. How he identifies whether a foreign DNA is inserted or not after cloning?

Rashid isolated a natural plasmid from a bacterium and is planning to facilitate cloning . a. What are the minimum requirements for considering the isolated plas-mid as a vector ? How he identifies whether a foreign DNA is inserted or not after clon-ing ?

Plasmid used to construct the first recombinant DNA was isolated from which bacterium species?

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

Read the following statements and select the correct ones. (i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes. (ii) Exonucleases make cuts at specific positions within the DNA. (iii) Hind ll was the first restriction endonuclease to be isolated. (iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA. (v) Presence of more than one recognition sites for a enzyme within the vector facilitates the gene cloning.

Read the following statements and select the correct ones. (i) Electrophoresis is a technique used for the separation of molecules based on their size and charge. (ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double stranded DNA molecules found naturally in many bacteria and also in some yeast,. (iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule. (iv) In EcoRl, the roman numberal I indicates that it was the first enzyme isolated from E.coli A) (i) and (ii) B) (iii) and (iv) C) (i),(ii) and (iv) D) (i),(ii),(iii) and (iv)

Consider the following statements : i. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro ii. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid. iii. Restriction enzymes belong to a class of enzymes called nucleases . Choose that correct option regarding above statements