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A Danish Physician, Christian Gram devel...

A Danish Physician, Christian Gram developed a staining procedure to differentiate bacteria. List the various steps involved in that procedure.

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(a) (i) Gram staining Techniques :
1. Prepare a smear of bacterial culture
2. Stain with crystal violet for 30 seconds
3. Rinse in distilled water for 2 seconds
4. Grams lodine for 1 minute
5. Rinse in distilled water
6. Wash in `95%` ethanol or acetone for 10 to 30 seconds
7. Rinse in distilled water
8. Safranin for 30-60seconds
9. Rinse in distilled water and blot
10. Observe under microscope
(ii)
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