Explain Kuhne's experiment and the action of enzyme zymase.

Explanation

Demonstration of alcoholic fermentation :
Take a Kuhne's fermentation tube which consists of an upright glass tube with side bulb . Pour 10 % sugar solution mixed with baker's yeast into the fermentation tube the side tube is filled plug the mouth with lid . After some time , the glucose colution will be fermented . The solution willgive out an alcoholic smell and level of solution in glass column will fall due to the accumulation of `CO_(2)` gas . It is due to the presence of zymasa enzyme in yeast which converts the glucose solution into alcohol and `CO_(2)` . Now introduce a pellet of of KOH into the tube ,the KOH will absorb `CO_(2)` and the level of solution will risein upright tube .