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Describe the steps involved in cytologic...

Describe the steps involved in cytological techniques.

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There are different types of mounting based on the portion of a specimen to be observed.
1. Whole mount: The whole organism or smaller structure is mounted over a slide and observed.
2. Squash: Is a preparation where the material to be observed is crushed/ squashed onto a slide so as to reveal their contents. example: Pollen grains, mitosis and meiosis in root tips and flower buds to observe chromosomes.
3. Smears: here the specimen is in the fluid (blood and microbial cultures etc) are scraped, brushed or aspirated from surface of organ. example: Epithelial cells.
4. Sections: Free hand sections from a specimen and thin sections are selected, strained and mounted on a slide. example: Leaf and stem of plants.
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