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test is used as an indicator of the pres...

_____test is used as an indicator of the presence of protein.

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The biuret test is used as an indicator of the presence of protein because it gives a purple colour in the presence of peptide bonds (-C-N-). To a protein solution an equal quantity of sodium hydroxide solution is added and mixed. Then a few drops of 0.5% copper (II) sulphate is added with gentle mixing. a distinct purple colour developls without heating.
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